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No. 9 (2020): Sampling Protocols: Version 1

Sampling Protocols: Version 1

  • The Nansen Legacy
December 10, 2020


The collection of detailed sampling protocols is crucial tool for the success of the Nansen Legacy, because they ensure:

  • Methodological agreement between the involved researchers
  • Continuity and comparable data throughout the 5 years sampling period
  • An easily accessible overview over parameters sampled
  • Easier cruise planning


  1. Andersen, R.A. & Kawachi, M. 2005. Traditional microalgae isolation techniques. In: Andersen, R. A. (ed). Algal Culturing Techniques. Elsevier Academic Press, 578 p.
  2. Bollmann J, Cortes MY, Haidar AT, Brabec B, Close A, Hofmann R, et al. Techniques for quantitative analyses of calcareous marine phytoplankton. Mar Micropaleontol. 2002;44(3–4):163–85.
  3. Edvardsen et al. 2003. Genetic variability and molecular phylogeny of Dinophysis species (Dinophyceae) from Norwegian waters inferred from single cell analyses of rDNA. J. Phycol. 39: 395-408.
  4. Heino, M., Porteiro, F.M., Sutton, T.T., Falkenhaug, T., Godø, O.R., Piatkowski, U., 2011. Catchability of pelagic trawls for sampling deep-living nekton in the mid-North Atlantic. ICES Journal of Marine Science 68, 377–389.
  5. John, S, Poulos, B and Schirmer C. (2015). Ion Chloride Precipitation of viruses from seawater,
  6. Kirchman D (2001) Measuring bacterial biomass production and growth rates from leucine incorporation in natural aquatic environments. Methods in Microbiology. 30: 227-237.
  7. Kirchman DL (1993). Leucine incorporation as a measure of biomass production by heterotrophic bacteria. In: Current Methods in Aquatic Microbial Ecology (P. Kemp, BE Sherr EB Sherr and JJ Cole, Eds). Lewis Publishers, Boca Raton, EL.
  8. Krafft, B.A., Melle, W., Knutsen, T., Bagøien, E., Broms, C., Ellertsen, B., Siegel, V., 2010. Distribution and demography of Antarctic krill in the Southeast Atlantic sector of the Southern Ocean during the austral summer 2008. Polar Biology 33, 957–968. DOI: 10.1007/s00300-010-0774-3
  9. Matteson, A.R., Budinoff, C.R., Campbell, C.E., Buchan, A., Wilhelm, S.W. Estimating Virus Production Rates in Aquatic Systems. J. Vis. Exp. (43), e2196, doi: 10.3791/2196
  10. Melle, W., Abrahamsen, M., Valdemarsen, J.W., Ellertsen, B., Knutsen, T. 2006. Design and performance of a new macro-plankton trawl in combination with a multiple cod-end system. SCOR Working Group 115, Mini Symposium on Standards for the Survey and Analysis of Plankton. Plymouth, England. 19-20 May 2006.
  11. Simon M and Azam F (1989). Protein content and protein synthesis rates of planktonic marine bacteria. Mar. Ecol. Prog. Ser. 51: 201-213. DOI: 10.3354/meps051201
  12. Smith DC and Azam F (1992). A simple, economical method for measuring bacterial protein synthesis in seawater using 3H-leucine. Mar. Microb. Food Webs 6: 107-114.
  13. Šupraha L, Ljubešić Z, Mihanović H, Henderiks J. Coccolithophore life-cycle dynamics in a coastal Mediterranean ecosystem: Seasonality and species-specific patterns. J Plankton Res. 2016;38(5).
  14. Throndsen J (1995) Estimating cell numbers. In: GM Hallegraeff DM Anderson AD Cembella. Manual on Harmful Marine Microalgae. Paris: UNESCO. pp. 63-80.
  15. Utermöhl, H. (1958). Zur Vervollkommung der quantitativen Phytoplankton-Methodik. Mitt. Int. Ver. Theor. Angew. Limnol. 9, 1–38.
  16. Weinbauer, Rowe, Wilhelm, 2010, Determing rates of virus production in acualtic systems by the virus reduction approach., MAVE, chapter 1 pp 1-8. DOI: 10.4319/mave.2010.978-0-9845591-0-7.1
  17. Wenneck, T.dL., Falkenhaug T., Bergstad OA (2008). Strategies, methods, and technologies adopted on the R.V. G.O. Sars MAR-ECO expedition to the Mid-Atlantic Ridge in 2004. Deep Sea Research II. 55: 6-28.