Skip to main navigation menu Skip to main content Skip to site footer


No 10 (2020): Sampling Protocols: Version 2

Sampling Protocols: Version 2

  • The Nansen Legacy
December 18, 2020


The collection of detailed sampling protocols is crucial tool for the success of the Nansen Legacy, because they ensure:

  • Methodological agreement between the involved researchers
  • Continuity and comparable data throughout the 5 years sampling period
  • An easily accessible overview over parameters sampled
  • Easier cruise planning



  1. Andersen, R.A. & Kawachi, M. 2005. Traditional microalgae isolation techniques. In: Andersen, R. A. (ed). Algal Culturing Techniques. Elsevier Academic Press, 578 p.
  2. Edvardsen et al. 2003. Genetic variability and molecular phylogeny of Dinophysis species (Dinophyceae) from Norwegian waters inferred from single cell analyses of rDNA. J. Phycol. 39: 395-408.
  3. Heino, M., Porteiro, F.M., Sutton, T.T., Falkenhaug, T., Godø, O.R., Piatkowski, U., 2011. Catchability of pelagic trawls for sampling deep-living nekton in the mid-North Atlantic. ICES Journal of Marine Science 68, 377–389.
  4. ICES (2015). Manual for International Pelagic Surveys (IPS). Series of ICES Survey Protocols SISP 9 - IPS. 1–92.
  5. John, S, Poulos, B and Schirmer C. 2015, Ion Chloride Precipitation of viruses from seawater,
  6. Kirchman D (2001) Measuring bacterial biomass production and growth rates from leucine incorporation in natural aquatic environments. Methods in Microbiology. 30: 227-237.
  7. Kirchman DL (1993). Leucine incorporation as a measure of biomass production by heterotrophic bacteria. In: Current Methods in Aquatic Microbial Ecology (P. Kemp, BE Sherr EB Sherr and JJ Cole, Eds). Lewis Publishers, Boca Raton, EL.
  8. Korneliussen, R. J., Heggelund, Y., Macaulay, G. J., Patel, D., Johnsen, E., and Eliassen, I. K. (2016). Acoustic identification of marine species using a feature library. Methods Oceanogr. 17, 187–205. doi: 10.1016/j.mio.2016.09.002.
  9. Korneliussen, R. J., Ona, E., Eliassen, I., Heggelund, Y., Patel, R., God,ø, O. R., Giertsen, C., et al. (2006). “The large scale survey system - LSSS,” in Proceedings of the 29th Scandinavian Symposium on Physical Acoustics (Ustaoset).
  10. Krafft, B.A., Melle, W., Knutsen, T., Bagøien, E., Broms, C., Ellertsen, B., Siegel, V., 2010. Distribution and demography of Antarctic krill in the Southeast Atlantic sector of the Southern Ocean during the austral summer 2008. Polar Biology 33, 957–968. DOI: 10.1007/s00300-010-0774-3
  11. MacLennan, D. N., Fernandes, P. G., and Dalen, J. (2002). A consistent approach to definitions and symbols in fisheries acoustics. ICES J. Mar. Sci. 59, 365–369. doi: 10.1006/jmsc.2001.1158
  12. Marie, D., Shi, X.L., Rigaut-Jalabert, F. & Vaulot, D. (2010). Use of flow cytometric sorting to better assess the diversity of small photosynthetic eukaryotes in the English Channel. FEMS Microbiology Ecology. 72. p.pp. 165–178. DOI: 10.1111/j.1574-6941.2010.00842.x
  13. Matteson, A.R., Budinoff, C.R., Campbell, C.E., Buchan, A., Wilhelm, S.W. Estimating Virus Production Rates in Aquatic Systems. J. Vis. Exp. (43), e2196, DOI : 10.3791/2196 (2010).URL: Bollmann J, Cortes MY, Haidar AT, Brabec B, Close A, Hofmann R, et al. Techniques for quantitative analyses of calcareous marine phytoplankton. Mar Micropaleontol. 2002;44(3–4):163–85. DOI : 10.3791/2196 (2010).URL
  14. Melle, W., Abrahamsen, M., Valdemarsen, J.W., Ellertsen, B., Knutsen, T. 2006. Design and performance of a new macro-plankton trawl in combination with a multiple cod-end system. SCOR Working Group 115, Mini Symposium on Standards for the Survey and Analysis of Plankton. Plymouth, England. 19-20 May 2006.
  15. Molari, M., Manini, E., & Dell'Anno, A. (2013). Dark inorganic carbon fixation sustains the functioning of benthic deep‐sea ecosystems. Global Biogeochemical Cycles, 27(1), 212-221.
  16. Simon M and Azam F (1989). Protein content and protein synthesis rates of planktonic marine bacteria. Mar. Ecol. Prog. Ser. 51: 201-213. DOI: 10.3354/meps051201
  17. Smith DC and Azam F (1992). A simple, economical method for measuring bacterial protein synthesis in seawater using 3H-leucine. Mar. Microb. Food Webs 6: 107-114.
  18. Šupraha L, Ljubešić Z, Mihanović H, Henderiks J. Coccolithophore life-cycle dynamics in a coastal Mediterranean ecosystem: Seasonality and species-specific patterns. J Plankton Res. 2016;38(5).
  19. Throndsen J (1995) Estimating cell numbers. In: GM Hallegraeff DM Anderson AD Cembella. Manual on Harmful Marine Microalgae. Paris: UNESCO. pp. 63-80.
  20. Utermöhl, H. (1958). Zur Vervollkommung der quantitativen Phytoplankton-Methodik. Mitt. Int. Ver. Theor. Angew. Limnol. 9, 1–38.
  21. Vaulot, D. Tangential flow filtration (TFF) concentration of phytoplankton.
  22. Weinbauer, Rowe, Wilhelm, 2010, Determing rates of virus production in acualtic systems by the virus reduction approach., MAVE, chapter 1 pp 1-8. DOI: 10.4319/mave.2010.978-0-9845591-0-7.1
  23. Wenneck, T.dL., Falkenhaug T., Bergstad OA (2008). Strategies, methods, and technologies adopted on the R.V. G.O. Sars MAR-ECO expedition to the Mid-Atlantic Ridge in 2004. Deep Sea Research II. 55: 6-28.